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whole mouse genome microarray 4x44k v2  (Agilent technologies)


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    Agilent technologies whole mouse genome microarray 4x44k v2
    Whole Mouse Genome Microarray 4x44k V2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/whole+mouse+genome+4x44k+microarray/pmc10987564-257-0-0
    Average 90 stars, based on 1 article reviews
    whole mouse genome microarray 4x44k v2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Epigenetically mediated inhibition of S -adenosylhomocysteine hydrolase and the associated dysregulation of 1-carbon metabolism in nonalcoholic steatohepatitis and hepatocellular carcinoma
    Article Snippet: Total RNA was extracted from liver tissue samples of STAM mice and mice fed the CFD diet ( n = 5/group per treatment) using miRNeasy Mini kits (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions. .. Whole-genome hepatic gene expression profiles were determined with an Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray in the STAM mice and an Agilent-014868 Whole Mouse Genome 4x44K Microarray (Agilent Technologies, Santa Clara, CA, USA) in mice fed the CFD diet, as described in detail elsewhere ( 13 , 27 ). .. The microarray gene expression data have been deposited in the National Center for Biotechnology Information (NCBI; Bethesda, MD, USA) Gene Expression Omnibus database ( https://www.ncbi.nlm.nih.gov/geo/ ; accession numbers {"type":"entrez-geo","attrs":{"text":"GSE83596","term_id":"83596"}} GSE83596 and {"type":"entrez-geo","attrs":{"text":"GSE96936","term_id":"96936"}} GSE96936 ).

    Microarray:

    Article Title: Epigenetically mediated inhibition of S -adenosylhomocysteine hydrolase and the associated dysregulation of 1-carbon metabolism in nonalcoholic steatohepatitis and hepatocellular carcinoma
    Article Snippet: Total RNA was extracted from liver tissue samples of STAM mice and mice fed the CFD diet ( n = 5/group per treatment) using miRNeasy Mini kits (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions. .. Whole-genome hepatic gene expression profiles were determined with an Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray in the STAM mice and an Agilent-014868 Whole Mouse Genome 4x44K Microarray (Agilent Technologies, Santa Clara, CA, USA) in mice fed the CFD diet, as described in detail elsewhere ( 13 , 27 ). .. The microarray gene expression data have been deposited in the National Center for Biotechnology Information (NCBI; Bethesda, MD, USA) Gene Expression Omnibus database ( https://www.ncbi.nlm.nih.gov/geo/ ; accession numbers {"type":"entrez-geo","attrs":{"text":"GSE83596","term_id":"83596"}} GSE83596 and {"type":"entrez-geo","attrs":{"text":"GSE96936","term_id":"96936"}} GSE96936 ).

    Article Title: JNK1 ablation in mice confers long-term metabolic protection from diet-induced obesity at the cost of moderate skin oxidative damage
    Article Snippet: .. For microarray analysis, total RNAs were hybridized in quadruplicate on a whole mouse genome 4x44K microarray (G4122F; AMADID 014868; Agilent Technologies, Santa Clara, CA, USA), scanned with the Agilent microarray scanner at 5 μm resolution, and analyzed numerically with the manufacturer’s software. ..

    Software:

    Article Title: JNK1 ablation in mice confers long-term metabolic protection from diet-induced obesity at the cost of moderate skin oxidative damage
    Article Snippet: .. For microarray analysis, total RNAs were hybridized in quadruplicate on a whole mouse genome 4x44K microarray (G4122F; AMADID 014868; Agilent Technologies, Santa Clara, CA, USA), scanned with the Agilent microarray scanner at 5 μm resolution, and analyzed numerically with the manufacturer’s software. ..



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    A: Time course analysis of expression levels of the polycomb group (PcG) members (Ezh1, Eed, Suz12, Bmi1, Ring1B, and Ezh2) in CD45 − TER119 − non-hematopoietic liver cells of mice at nine time points between ED 9.5 and 8 weeks. Relative expression values from <t>microarray</t> analyses are shown. B: Western blot analysis of Ezh2 in CD45 − TER119 − non-hematopoietic liver cells of fetal mice at ED 11.5, 13.5, 15.5, and 17.5. β-actin is indicated as control. C: Immunofluorescence staining for Ezh2, CK8/18 (epithelial marker), and DAPI in liver of fetuses at ED 11.5, 13.5, 15.5, 17.5, P 0 (neonatal), and 8 weeks. Arrows indicate Ezh2 positive cells in CK8/18 positive cells. (Scale bar: 100 µm) D: Frequency of Ezh2 positive cells in CK8/18 positive cells at indicated time points. (n = 3). E: Immunofluorescence staining for histone H3K27me3, CK8/18 (epithelial marker), DAPI in the liver tissues at ED 11.5, 13.5, 15.5, and 17.5. Scale bar = 100 µm. F: Frequency of H3K27me3 expressing cells in CK8/18 positive cells at indicated points. Data are mean ± SD (n = 3). G Immunofluorescence staining for Ezh2, H3K27me3, Ck8/18 and DAPI in the liver of fetuses at ED 13.5. Upper is merge image of all colors. Bottom column shows image of Ezh2 and H3K27me3, lower right is magnified image of left. Red: Ezh2, Green: H3K27me3, Light blue: CK8/18, Blue: DAPI. Scale bar = 100 µm. H Immunofluorescence staining for AFP, Ezh2, CK8/18, and DAPI in the liver of fetuses at ED 13.5. Arrows indicate AFP and Ezh2 double positive cells in CK8/18 positive cells. (Scale bar: 50 µm) I: Frequency of Ezh2 positive cells in AFP and CK8/18 double positive cells and AFP negative and CK8/18 positive cells at indicated time points. (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05.
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    A: Time course analysis of expression levels of the polycomb group (PcG) members (Ezh1, Eed, Suz12, Bmi1, Ring1B, and Ezh2) in CD45 − TER119 − non-hematopoietic liver cells of mice at nine time points between ED 9.5 and 8 weeks. Relative expression values from <t>microarray</t> analyses are shown. B: Western blot analysis of Ezh2 in CD45 − TER119 − non-hematopoietic liver cells of fetal mice at ED 11.5, 13.5, 15.5, and 17.5. β-actin is indicated as control. C: Immunofluorescence staining for Ezh2, CK8/18 (epithelial marker), and DAPI in liver of fetuses at ED 11.5, 13.5, 15.5, 17.5, P 0 (neonatal), and 8 weeks. Arrows indicate Ezh2 positive cells in CK8/18 positive cells. (Scale bar: 100 µm) D: Frequency of Ezh2 positive cells in CK8/18 positive cells at indicated time points. (n = 3). E: Immunofluorescence staining for histone H3K27me3, CK8/18 (epithelial marker), DAPI in the liver tissues at ED 11.5, 13.5, 15.5, and 17.5. Scale bar = 100 µm. F: Frequency of H3K27me3 expressing cells in CK8/18 positive cells at indicated points. Data are mean ± SD (n = 3). G Immunofluorescence staining for Ezh2, H3K27me3, Ck8/18 and DAPI in the liver of fetuses at ED 13.5. Upper is merge image of all colors. Bottom column shows image of Ezh2 and H3K27me3, lower right is magnified image of left. Red: Ezh2, Green: H3K27me3, Light blue: CK8/18, Blue: DAPI. Scale bar = 100 µm. H Immunofluorescence staining for AFP, Ezh2, CK8/18, and DAPI in the liver of fetuses at ED 13.5. Arrows indicate AFP and Ezh2 double positive cells in CK8/18 positive cells. (Scale bar: 50 µm) I: Frequency of Ezh2 positive cells in AFP and CK8/18 double positive cells and AFP negative and CK8/18 positive cells at indicated time points. (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05.
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    A: Time course analysis of expression levels of the polycomb group (PcG) members (Ezh1, Eed, Suz12, Bmi1, Ring1B, and Ezh2) in CD45 − TER119 − non-hematopoietic liver cells of mice at nine time points between ED 9.5 and 8 weeks. Relative expression values from <t>microarray</t> analyses are shown. B: Western blot analysis of Ezh2 in CD45 − TER119 − non-hematopoietic liver cells of fetal mice at ED 11.5, 13.5, 15.5, and 17.5. β-actin is indicated as control. C: Immunofluorescence staining for Ezh2, CK8/18 (epithelial marker), and DAPI in liver of fetuses at ED 11.5, 13.5, 15.5, 17.5, P 0 (neonatal), and 8 weeks. Arrows indicate Ezh2 positive cells in CK8/18 positive cells. (Scale bar: 100 µm) D: Frequency of Ezh2 positive cells in CK8/18 positive cells at indicated time points. (n = 3). E: Immunofluorescence staining for histone H3K27me3, CK8/18 (epithelial marker), DAPI in the liver tissues at ED 11.5, 13.5, 15.5, and 17.5. Scale bar = 100 µm. F: Frequency of H3K27me3 expressing cells in CK8/18 positive cells at indicated points. Data are mean ± SD (n = 3). G Immunofluorescence staining for Ezh2, H3K27me3, Ck8/18 and DAPI in the liver of fetuses at ED 13.5. Upper is merge image of all colors. Bottom column shows image of Ezh2 and H3K27me3, lower right is magnified image of left. Red: Ezh2, Green: H3K27me3, Light blue: CK8/18, Blue: DAPI. Scale bar = 100 µm. H Immunofluorescence staining for AFP, Ezh2, CK8/18, and DAPI in the liver of fetuses at ED 13.5. Arrows indicate AFP and Ezh2 double positive cells in CK8/18 positive cells. (Scale bar: 50 µm) I: Frequency of Ezh2 positive cells in AFP and CK8/18 double positive cells and AFP negative and CK8/18 positive cells at indicated time points. (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05.
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    A: Time course analysis of expression levels of the polycomb group (PcG) members (Ezh1, Eed, Suz12, Bmi1, Ring1B, and Ezh2) in CD45 − TER119 − non-hematopoietic liver cells of mice at nine time points between ED 9.5 and 8 weeks. Relative expression values from <t>microarray</t> analyses are shown. B: Western blot analysis of Ezh2 in CD45 − TER119 − non-hematopoietic liver cells of fetal mice at ED 11.5, 13.5, 15.5, and 17.5. β-actin is indicated as control. C: Immunofluorescence staining for Ezh2, CK8/18 (epithelial marker), and DAPI in liver of fetuses at ED 11.5, 13.5, 15.5, 17.5, P 0 (neonatal), and 8 weeks. Arrows indicate Ezh2 positive cells in CK8/18 positive cells. (Scale bar: 100 µm) D: Frequency of Ezh2 positive cells in CK8/18 positive cells at indicated time points. (n = 3). E: Immunofluorescence staining for histone H3K27me3, CK8/18 (epithelial marker), DAPI in the liver tissues at ED 11.5, 13.5, 15.5, and 17.5. Scale bar = 100 µm. F: Frequency of H3K27me3 expressing cells in CK8/18 positive cells at indicated points. Data are mean ± SD (n = 3). G Immunofluorescence staining for Ezh2, H3K27me3, Ck8/18 and DAPI in the liver of fetuses at ED 13.5. Upper is merge image of all colors. Bottom column shows image of Ezh2 and H3K27me3, lower right is magnified image of left. Red: Ezh2, Green: H3K27me3, Light blue: CK8/18, Blue: DAPI. Scale bar = 100 µm. H Immunofluorescence staining for AFP, Ezh2, CK8/18, and DAPI in the liver of fetuses at ED 13.5. Arrows indicate AFP and Ezh2 double positive cells in CK8/18 positive cells. (Scale bar: 50 µm) I: Frequency of Ezh2 positive cells in AFP and CK8/18 double positive cells and AFP negative and CK8/18 positive cells at indicated time points. (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05.
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    Image Search Results


    A: Time course analysis of expression levels of the polycomb group (PcG) members (Ezh1, Eed, Suz12, Bmi1, Ring1B, and Ezh2) in CD45 − TER119 − non-hematopoietic liver cells of mice at nine time points between ED 9.5 and 8 weeks. Relative expression values from microarray analyses are shown. B: Western blot analysis of Ezh2 in CD45 − TER119 − non-hematopoietic liver cells of fetal mice at ED 11.5, 13.5, 15.5, and 17.5. β-actin is indicated as control. C: Immunofluorescence staining for Ezh2, CK8/18 (epithelial marker), and DAPI in liver of fetuses at ED 11.5, 13.5, 15.5, 17.5, P 0 (neonatal), and 8 weeks. Arrows indicate Ezh2 positive cells in CK8/18 positive cells. (Scale bar: 100 µm) D: Frequency of Ezh2 positive cells in CK8/18 positive cells at indicated time points. (n = 3). E: Immunofluorescence staining for histone H3K27me3, CK8/18 (epithelial marker), DAPI in the liver tissues at ED 11.5, 13.5, 15.5, and 17.5. Scale bar = 100 µm. F: Frequency of H3K27me3 expressing cells in CK8/18 positive cells at indicated points. Data are mean ± SD (n = 3). G Immunofluorescence staining for Ezh2, H3K27me3, Ck8/18 and DAPI in the liver of fetuses at ED 13.5. Upper is merge image of all colors. Bottom column shows image of Ezh2 and H3K27me3, lower right is magnified image of left. Red: Ezh2, Green: H3K27me3, Light blue: CK8/18, Blue: DAPI. Scale bar = 100 µm. H Immunofluorescence staining for AFP, Ezh2, CK8/18, and DAPI in the liver of fetuses at ED 13.5. Arrows indicate AFP and Ezh2 double positive cells in CK8/18 positive cells. (Scale bar: 50 µm) I: Frequency of Ezh2 positive cells in AFP and CK8/18 double positive cells and AFP negative and CK8/18 positive cells at indicated time points. (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05.

    Journal: PLoS ONE

    Article Title: Polycomb Group Protein Ezh2 Regulates Hepatic Progenitor Cell Proliferation and Differentiation in Murine Embryonic Liver

    doi: 10.1371/journal.pone.0104776

    Figure Lengend Snippet: A: Time course analysis of expression levels of the polycomb group (PcG) members (Ezh1, Eed, Suz12, Bmi1, Ring1B, and Ezh2) in CD45 − TER119 − non-hematopoietic liver cells of mice at nine time points between ED 9.5 and 8 weeks. Relative expression values from microarray analyses are shown. B: Western blot analysis of Ezh2 in CD45 − TER119 − non-hematopoietic liver cells of fetal mice at ED 11.5, 13.5, 15.5, and 17.5. β-actin is indicated as control. C: Immunofluorescence staining for Ezh2, CK8/18 (epithelial marker), and DAPI in liver of fetuses at ED 11.5, 13.5, 15.5, 17.5, P 0 (neonatal), and 8 weeks. Arrows indicate Ezh2 positive cells in CK8/18 positive cells. (Scale bar: 100 µm) D: Frequency of Ezh2 positive cells in CK8/18 positive cells at indicated time points. (n = 3). E: Immunofluorescence staining for histone H3K27me3, CK8/18 (epithelial marker), DAPI in the liver tissues at ED 11.5, 13.5, 15.5, and 17.5. Scale bar = 100 µm. F: Frequency of H3K27me3 expressing cells in CK8/18 positive cells at indicated points. Data are mean ± SD (n = 3). G Immunofluorescence staining for Ezh2, H3K27me3, Ck8/18 and DAPI in the liver of fetuses at ED 13.5. Upper is merge image of all colors. Bottom column shows image of Ezh2 and H3K27me3, lower right is magnified image of left. Red: Ezh2, Green: H3K27me3, Light blue: CK8/18, Blue: DAPI. Scale bar = 100 µm. H Immunofluorescence staining for AFP, Ezh2, CK8/18, and DAPI in the liver of fetuses at ED 13.5. Arrows indicate AFP and Ezh2 double positive cells in CK8/18 positive cells. (Scale bar: 50 µm) I: Frequency of Ezh2 positive cells in AFP and CK8/18 double positive cells and AFP negative and CK8/18 positive cells at indicated time points. (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05.

    Article Snippet: Expression profiling was obtained with Whole Mouse Genome 4x44K v2 OligoDNA Microarray Kit (Agilent Technologies) according to manufacturer's instruction.

    Techniques: Expressing, Microarray, Western Blot, Immunofluorescence, Staining, Marker, MANN-WHITNEY

    A: Western blot analysis for Albumin (hepatocyte marker) in the CD45 − TER119 − non-hematopoietic liver cells from control (Rosa26::CreER(T2) −/− ;Ezh2 F/F mouse) and Ezh2 KO (Ezh2 SET domain depleted mouse; Rosa26::CreER(T2) +/− ;Ezh2 F/F mouse) at ED 18.5 after 3 days TAM injection (TAM; ED 10.5–12.5). B: Expression levels of hepatocytes related genes in the CD45 − TER119 − non-hematopoietic liver cells of the control and Ezh2 KO at indicated points were measured by qRT-PCR. Data are mean ± SD (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05. C: The heat map generated from microarray analysis for the liver development signature 83-gene expression in the CD45 − TER119 − non-hematopoietic liver cells of the control and Ezh2 KO. D: Significant enrichment of metabolism related liver functional GO terms for decreased genes upon Ezh2KO. Corrected P values of GO terms are shown.

    Journal: PLoS ONE

    Article Title: Polycomb Group Protein Ezh2 Regulates Hepatic Progenitor Cell Proliferation and Differentiation in Murine Embryonic Liver

    doi: 10.1371/journal.pone.0104776

    Figure Lengend Snippet: A: Western blot analysis for Albumin (hepatocyte marker) in the CD45 − TER119 − non-hematopoietic liver cells from control (Rosa26::CreER(T2) −/− ;Ezh2 F/F mouse) and Ezh2 KO (Ezh2 SET domain depleted mouse; Rosa26::CreER(T2) +/− ;Ezh2 F/F mouse) at ED 18.5 after 3 days TAM injection (TAM; ED 10.5–12.5). B: Expression levels of hepatocytes related genes in the CD45 − TER119 − non-hematopoietic liver cells of the control and Ezh2 KO at indicated points were measured by qRT-PCR. Data are mean ± SD (n = 3). P values (asterisks) are from the Mann–Whitney U -test. * P <0.05. C: The heat map generated from microarray analysis for the liver development signature 83-gene expression in the CD45 − TER119 − non-hematopoietic liver cells of the control and Ezh2 KO. D: Significant enrichment of metabolism related liver functional GO terms for decreased genes upon Ezh2KO. Corrected P values of GO terms are shown.

    Article Snippet: Expression profiling was obtained with Whole Mouse Genome 4x44K v2 OligoDNA Microarray Kit (Agilent Technologies) according to manufacturer's instruction.

    Techniques: Western Blot, Marker, Injection, Expressing, Quantitative RT-PCR, MANN-WHITNEY, Generated, Microarray, Functional Assay